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. 2021 Jan 1;11(4):1763–1779. doi: 10.7150/thno.47057

Figure 4.

Figure 4

HnRNPA2B1 mediates the packaging of miR-30b-3p into EVs. (A) qRT-PCR analysis of miR-30b-3p in EVs from GSC664 and GSC712 cells treated with shHIF1α or shSTAT3. Six replicates per group, three independent experiments per group. (B) qRT-PCR analysis of miR-30b-5p in EVs from GSC664 and GSC712 cells treated with shHIF1α or shSTAT3. Six replicates per group, three independent experiments per group. (C) qRT-PCR analysis of miR-30b-3p in normoxic and hypoxic EVs from GSC664 and GSC712 cells transfected with si-Ctrl or si-hnRNPA2B1. Six replicates per group, three independent experiments per group. (D) qRT-PCR analysis of miR-30b-3p in normoxic and hypoxic EVs from GSC664 and GSC712 cells transfected with vector or hnRNPA2B1. Six replicates per group, three independent experiments per group. (E) GSC664 and GSC712 cells were subjected to RIP assay using an anti-hnRNPA2B1 antibody or IgG. qRT-PCR was used to analyze miR-30b-3p enrichment in IP-enriched RNA. Six replicates per group, three independent experiments per group. (F) Immunoblotting for hnRNPA2B1 in samples pulled down with WT miR-30b-3p, Mut miR-30b-3p or no probe. Three replicates per group, three independent experiments per group. (G) Relative miR-30b-3p levels in IP products of hnRNPA2B1 from normoxic or hypoxic GSCs. Three replicates per group, three independent experiments per group, **P < 0.01.