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. 2021 Jan 1;11(4):1991–2005. doi: 10.7150/thno.50613

Figure 2.

Figure 2

3D collagen I/FN gel induces tumor progression by integrin αvβ3 (A) Tumorigenesis of LN229 cells cultured in a flask or 3D fibrin gel in the NSG mouse (n=6). mRNA expression of integrin beta (B) or alpha (C) in LN229 cells cultured in a flask or 3D Collagen/FN gel for 48h. (D) Expression of integrin αvβ3 in LN229 cells cultured in a flask or 3D Collagen/FN gel for 48h. (E) Representative photographs of 3D collagen/FN cultured LN229 cells treated with PBS or SB273005 (5 nM). Scale bar represents 20 µm. (F) LN229 cells cultured in 3D collagen/FN gel were treated with PBS or SB273005(5 nM). Quantification of colony size is shown (n = 10). (G) Cell viability of 3D collagen/FN cultured LN229 cells treated with PBS or SB273005 (5 nM). (H) Colony formation analysis of 3D collagen/FN-cultured LN229 cells, treated with PBS or SB273005 (5 nM). (I) Tumorigenesis of 3D collagen/FN-cultured LN229 cells, treated with PBS or SB273005 (5 nM) (n=6). (J) Immunofluorescence staining of integrin αvβ3 in HM and LM glioma tissues. Scale bar represents 50 µm. (K) Representative photographs of 3D collagen/FN-cultured LN229-NC and LN229- ITGB3 KO cells. (L) Colony sizes of 3D collagen/FN-cultured LN229-NC and LN229- ITGB3 KO cells. (M) Cell proliferation of 3D collagen/FN-cultured LN229-NC and LN229- ITGB3 KO cells. (N) Colony formation of 3D collagen/FN cultured LN229-NC and LN229-ITGB3 KO cells. (O) Tumorigenesis of 3D collagen/FN cultured LN229-NC and LN229-ITGB3 KO cells. Mean ± SEM, n.s, no significant difference, *p < 0.05, **p < 0.01.