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. 2021 Jan 1;11(4):1594–1608. doi: 10.7150/thno.48067

Figure 3.

Figure 3

Lack of positive selection of CD4+CD8+ splenic Tg8 cells. A) Spleen, and thymus tissues from 7-week-old Tg8 animals were homogenized and stained. Cells were gated for CD3 expression, and CD3+ cells analyzed for CD4, CD8 and CD69 expression. The y-axis shows the percentage of CD69+ cells among all double-positive (DP) CD4+CD8+ cells. Data are the mean ± SEM (n = 5 mice per genotype). B) Splenic CD4 single-positive (4SP) cells from Tg8 Thy1.1 animals and CD4+CD8+ (DP) cells from 7-week-old Tg8 Thy1.1/1.2 animals were sorted separately and co-injected in spleen (top panels) or thymus (bottom panels) of Tg8 Thy1.2 recipient animals. One week later, cells were collected from the spleen or thymus of recipient mice, and stained with anti-CD4, -CD8, -Thy1.1 and -Thy1.2 antibodies, cells were positively gated in Thy1.1 for donor cells or gated Thy1.2 host cells, and analyzed for CD4 and CD8. Data are representative of n = 5 mice per group. ** p < 0.01 (Mann-Whitney test).