Figure 4.

Occurrence of intravascular cell masses, occlusions, and blood flow arrest, by blood vessel type. (A) Number of blood vessel segments observed across all animals ('Total Vessels'), FUS+MB affected blood vessels (i.e. exhibited increases in BBB permeability, 'Affected Vessels'), blood vessels with a 'Cell Mass/Occlusion', and blood vessels exhibiting 'Blood Flow Arrest' observed, grouped by blood vessel type, and (B) relative to the time point at which increases in BBB permeability were observed ('Pre' or 'Post'). (C) Occurrences of cell masses and/or occlusions in affected blood vessels are shown in green, compared to all blood vessels in which BBB leakage was observed (black). Examples of (D) cell masses, (E) cell occlusion, and (F) blood flow arrest, here observed as stalled erythrocytes (dark shapes). The duration for which cell masses, cell occlusions, and blood flow arrest were observed were grouped as 'transient' (seconds) or 'persistent' (minutes), shown in B. Time indicated is relative to the onset of FUS exposure. Blood vessel are shown in red (Texas Red dextran), and EGFP+ fluorescence cells are shown in green. Scale bars: 50 µm.