Skip to main content
. 2021 Jan 1;11(4):1780–1794. doi: 10.7150/thno.51931

Figure 1.

Figure 1

Antisense eRNA is negatively related with antisense mRNA on AR target loci. (A) Screen shots from UCSC genome browser showing signal profiles of eRNA and mRNA expression in LNCaP. ChIP-seq in LNCaP and C4-2 were shown as a reference. PSA (KLK3) was in left panel; FKBP5 was in right panel. The enhancer regions were highlighted in yellow box. (B) Sense eRNA, sense mRNA, antisense eRNA (as-eRNA) and antisense ncRNA expressions of PSA were measured by qRT-PCR in C4-2 cells treated with DHT. Means and standard deviations (error bar) were determined from three replicates. Error bars represented mean ± SD for triplicate experiments. P values were shown in the figures. GAPDH as internal control. (C) Sense eRNA, sense mRNA, antisense eRNA (as-eRNA) and antisense ncRNA expressions of PSA were measured by qRT-PCR in C4-2 cells treated with enzalutamide (ENZ). Means and standard deviations (error bar) were determined from three replicates. Error bars represented mean ± SD for triplicate experiments. P values were shown in the figures. GAPDH as internal control. (D) Sense eRNA, sense mRNA, antisense eRNA (as-eRNA) and antisense ncRNA expressions of PSA were measured by qRT-PCR in C4-2 cells knocked down with antisense eRNA LNAs. Means and standard deviations (error bar) were determined from three replicates. Error bars represented mean ± SD for triplicate experiments. P values were shown in the figures. GAPDH as internal control.