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. 2021 Jan 1;11(4):1828–1844. doi: 10.7150/thno.48698

Figure 3.

Figure 3

ARF1 promotes colon tumorigenesis by inducing ERK-mediated mitochondrial fission in vitro and in vivo. (A) Successful knockdown of ARF1 in HT29 and DLD1 cells. (B-C) Effect of ARF1 knockdown on HT29 and DLD1 cell proliferation (B) and colony formation (C). (D) Comparison of the morphology of mitochondria in ARF1-knockdown HT29 and DLD1 cells and control cells. (E) Effect of manipulating ARF1 expression on p-ERK and p-Drp1 expression levels. (F) Images of tumors and tumor curves showing significant growth inhibition of HT29- and DLD1-derived tumor xenografts (n = 6) by ARF1 knockdown. (G) Quantification of Ki-67 proliferation index in tumors. (H) Immunohistochemical analysis of p-ERK expression in tumors. (I) p-Drp1 and p-ERK expression in tumors. Bars, SD; *, P < 0.05; **, P < 0.01; ***, P < 0.001.