Figure 1.

Schematic of screening Fc variants by mammalian display. Fc variants libraries were designed based on Fc/FcγR complex structure and cloned into lentiviral vector. The cells were transduced with Fc variant library and stained by biotinylated FcγR protein and streptavidin-PE. The cells with high FcγR binding were sorted by FACS and cultured. After iterative rounds of selection, the Fc variant genes were retrieved by PCR and the abundance of variants was quantitated by NGS. FACS, fluorescence-activated cell sorting; NGS, next generation sequencing; PE, Phycoerythrin.