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. 2021 Jan 1;11(4):1901–1917. doi: 10.7150/thno.51299

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Display of Fc or its variants by FUT8 knockout CHO-K1 cells. (A) Schematic of CRISPR/Cas9-mediated FUT8 knockout in CHO-K1 cells. Three different sgRNAs were designed to target the 10^th exon of FUT8 as shown in red box. (B) Wild-type Fc or Fc variants were displayed on the FUT8 expressing CHO-K1 or FUT8^-/- CHO-K1 cells. The cells were stained with FITC conjugated anti-FLAG antibody, biotinylated FcγRIIIa protein and streptavidin-PE and analyzed by flow cytometry. (C) Oligosaccharide profiles of trastuzumab variants. (D) ADCC assay of fucosylated or defucosylated trastuzumab or trastuzumab variants using SK-BR-3 as target cells and PBMC as effector cells. Bars represent the mean ± SEM of three independent experiments.