Table 3.
KD and KD(FcγRIIb)/KD(FcγRIIa) ratios of NK003 variants
| NK003 variants | KD for FcγRI (mol/L) |
KD for FcγRIIb (mol/L) |
KD for FcγRIIIaF158 (mol/L) |
KD for FcγRIIIaV158 (mol/L) |
KD for FcγRIIaH131 (mol/L) |
KD for FcγRIIaR131 (mol/L) |
KD for FcRn pH 6 (mol/L) |
KD for FcRn pH 7.4 (mol/L) |
KD(FcγRIIb)/ KD(FcγRIIaH131) |
KD(FcγRIIb)/ KD(FcγRIIaR131) |
|---|---|---|---|---|---|---|---|---|---|---|
| WT | 1.04E-08 | 6.26E-06 | 2.12E-06 | 5.95E-07 | 1.39E-06 | 2.29E-06 | 3.77E-08 | NB | 4.50 | 2.73 |
| V266I/S267E/ K326V/L328A |
9.43E-09 | 3.69E-08 | 8.16E-07 | 4.14E-07 | 7.65E-07 | 2.11E-08 | 5.32E-08 | NB | 0.05 | 1.75 |
| G236D/ V266I/S267E/ K326V/L328A |
1.84E-08 | 1.82E-08 | 4.66E-06 | 4.98E-06 | 1.40E-06 | 1.94E-08 | 5.40E-08 | NB | 0.01 | 0.94 |
| P238D/ V266I/S267E/ K326V/L328A |
1.78E-08 | 1.08E-06 | WB | NB | NB | 1.98E-06 | 5.86E-08 | NB | / | 0.55 |
| S267E/L328F | 8.78E-09 | 2.39E-08 | 4.24E-06 | 5.22E-06 | 1.45E-06 | 1.30E-08 | 3.91E-08 | NB | 0.02 | 1.84 |
NB, no measurable binding; WB, weak binding.