Table 1.

List of identified NHXs with their accession IDs (from Gramene database), Chromosomal location and strand, size (aa: amino acid), Iso-electric point (pI), molecular weight (MW) (kDa), instabilty index, aliphatic Index, GRAVY, sub-cellular localization (V: Vacuole) and transmembrane helixes (predicted no., number of aa in TMH)

Gene	Accession ID	Chromosomal location and strand (±)	Size (aa)	pI	MW (kDa)	Instability Index	Aliphatic Index	GRAVY	Sub-cellular Localization	Predicted TMHs	aas in TMHs
GmNHX1	GLYMA_03G243000	Chr3:44,079,352-44,085,682 (-)	547	8.77	60.79	34.44	108.14	0.466	V	12	251.54
GmNHX2	GLYMA_07G270400	Chr7:44,306,021-44,315,600(-)	455	6.20	50.05	40.34	112.26	0.734	V	09	229.41
GmNHX3	GLYMA_08G324200	Chr8:44,233,968-44,242,320(-)	527	7.69	58.72	37.41	118.37	0.645	V	10	234.59
GmNHX4	GLYMA_09G018200	Chr9:1,420,668-1,431,498(-)	534	5.38	58.45	39.20	102.64	0.460	V	10	239.39
GmNHX5	GLYMA_10G158700	Chr10:39,253,048-39,262,228(-)	546	6.78	60.12	36.47	113.52	0.558	V	10	232.14
GmNHX6	GLYMA_15G124100	Chr15:9,849,463-9,859,551(-)	534	5.53	58.39	40.62	102.66	0.475	V	10	240.26
GmNHX7	GLYMA_17G022500	Chr17:1,643,783-1,649,939(-)	536	9.06	59.64	37.88	116.92	0.626	V	11	234.36
GmNHX8	GLYMA_19G240500	Chr19:48,830,897-48,836,752(-)	547	8.99	60.74	36.10	110.46	0.474	V	12	253.82
GmNHX9	GLYMA_20G229900	Chr20:46,409,048-46,417,710(+)	546	6.78	60.10	36.66	112.45	0.531	V	10	233.17