Fig. 5.

Substrate specificity and saturation curve of the purified recombinant M. timonae CTI-57 α-amylase AMY1. a Starch, β-cyclodextrin (β-CD), amylopectin, and maltodextrin at 0.5% were used in the DNS reaction method. b The substrates Bp-NPG7 and p-NPG were evaluated regarding p-NP release in a reaction. c Saturation curve varying starch concentration from 0.2 to 2.0 mg/mL. The initial velocity was measured as mg of starch that is degraded per min. All data represent the average and SD of three experimental sets