KEY RESOURCE TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Brilliant Violet 605 anti-mouse CD45 | BioLegend | Cat#103140 |
| PE/Cyanine7 anti-mouse CD31 | BioLegend | Cat#102418 |
| APC anti-mouse CD140a | BioLegend | Cat#135908 |
| PerCP/Cyanine5.5 anti-mouse Ly-6A/E (Sca-1) | BioLegend | Cat#108124 |
| PE anti-mouse CD106 (Vcam1) | BioLegend | Cat#105713 |
| Anti-MyHC | Sigma | Cat# |
| RNAscope® 4-Plex Negative Control Probe | Advanced Cell Diagnostics | Cat#321831 |
| RNAscope® 4-Plex Positive Control Probe-Mm | Advanced Cell Diagnostics | Cat#321811 |
| RNAscope Probe - Mm-Sucnr1-C3 | Advanced Cell Diagnostics | Cat#437721-C3 |
| RNAscope Probe - Mm-Des | Advanced Cell Diagnostics | Cat#407921 |
| RNAscope Probe - Mm-Pax7-C2 | Advanced Cell Diagnostics | Cat#314181-C2 |
| RNAscope Probe - Mm-Pdgfra-C4 | Advanced Cell Diagnostics | Cat#480661-C4 |
| Opal 520 | Akoya Biosciences | Cat#FP1487001KT |
| Opal 570 | Akoya Biosciences | Cat#FP1488001KT |
| Opal 620 | Akoya Biosciences | Cat#FP1495001KT |
| Opal 690 | Akoya Biosciences | Cat#FP1497001KT |
| anti-Tubulin β-3 | BioLegend | Cat#802001 |
| LEAF Purified anti-mouse CD31 | BioLegend | Cat#102512 |
| Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 | Invitrogen | Cat#A21245 |
| Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 | Invitrogen | Cat#A11077 |
| Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 | Invitrogen | Cat#A11001 |
| Streptavidin MicroBeads | Miltenyi Biotec | Cat#130-048-101 |
| SA-Texas Red | Invitrogen | Cat#S872 |
| Biotin rat anti-mouse CD45 | BD Biosciences | Cat#553078 |
| Biotin rat anti-CD11b | BD Biosciences | Cat#553309 |
| CD31 monoclonal antibody | eBiosciences | Cat#13-0311-82 |
| Biotin rat anti-mouse Ly-6A/E | BD Biosciences | Cat#553334 |
| PE anti-integrin α7 antibody | AbLab | Cat#10ST215 |
| CD34 monoclonal antibody eFluor 660 | eBioscience | Cat#50-0341-82 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| DMEM | Corning | Cat#10-017-CV |
| Fetal Bovine Serum | Gemini Bio-Products | Cat#100-106 |
| Penicillin streptomycin | Fisher scientific | Cat#15-140-163 |
| Horse Serum, New Zealand origin | Fisher scientific | Cat#16050130 |
| Collagenase II | Worthington Biochemical Corporation | Cat#LS004176 |
| Natural Protease (Dispase) | Worthington Biochemical | Cat#LS02104 |
| Thymine-d4 | Cambridge Isotope | Cat#DLM-1089 |
| Inosine-15N4 | Cambridge Isotope | Cat#NLM-4264 |
| Glycocholate-d4 | Cambridge Isotope | Cat#DLM-2742 |
| Methanol | Fisher Scientific | Cat# A4124 |
| Ammonium acetate | Sigma | Cat#372331-100G |
| Ammonium hydroxide | Sigma | Cat# 338818-100ML |
| Acetonitrile | Fisher Scientific | Cat# A9964 |
| l-glutamine | Sigma | Cat#W368401 |
| HEPES | Sigma | Cat#H0887 |
| Pyruvate | Sigma | Cat#P2256 |
| Glucose | Sigma | Cat#G7021 |
| Atpenin A5 | Cayman Chemical | Cat#11898 |
| Antimycin | Sigma | Cat#A8674 |
| Monensin | Sigma | Cat#M5273 |
| Ammonium chloride | Sigma | Cat#254134 |
| EIPA | Sigma | Cat#337850 |
| BCECF-AM | Invitrogen | Cat#B1170 |
| Nigericin | VWR | Cat#89156-870 |
| α-cyano-4-hydroxycinnamic acid | Sigma | Cat#C2020 |
| AZD 3965 | Fisher Scientific | Cat#501364664 |
| ARC155858 | EMD Millipore | Cat#533436 |
| Opti-MEM | ThermoFisher Scientific | Cat#31985070 |
| Lipofectamine RNAiMAX | Invitrogen | Cat#13778-150 |
| Sodium Chloride | Fisher Scientific | Cat#S2711 |
| Potassium Chloride | Sigma | Cat#P9333 |
| Calcium Chloride | Sigma | Cat#499609 |
| Magnesium Chloride | Sigma | Cat#M8266 |
| Sodium Phosphate Dibasic | Sigma | Cat#S9763 |
| [U13C] succinate | Cambridge Isotopes | Cat#CLM-1571 |
| [U13C] lactate | Cambridge Isotopes | Cat#CLM-1578 |
| cOmplete Protease Inhibitor Cocktail | Sigma-Aldrich | Cat#CO-RO |
| PhosSTOP, Phosphatase Inhibitor | Sigma-Aldrich | Cat#PHOSS-RO |
| EPPS | Sigma-Aldrich | Cat#E9502 |
| Bond-Breaker TCEP Solution, Neutral pH | ThermoFisher Scientific | Cat#77720 |
| Iodoacetamide | Sigma-Aldrich | Cat#I1149 |
| Dithiothreitol (DTT) | Sigma-Aldrich | Cat#DTT-RO |
| Lambda phosphatase | Santa Cruz Biotechnology | Cat#sc-200312 |
| Lys-C | Wako Chemicals | Cat#125-05061 |
| Trypsin | Promega | Cat#V5113 |
| 16-plex TMT reagents | Thermo Fisher Scientific | Cat#A44520 |
| DMEM, high glucose, no glutamine, no phenol red | Life Technologies | Cat#31053028 |
| Collagenase VIII | Sigma | Cat#C2139 |
| Dispase II | Thermo Fisher Scientific | Cat#17105041 |
| Triton X-100 | Sigma | Cat#X100 |
| Bovine Serum Albumin | Sigma | Cat#A9647 |
| Trypan Blue | Sigma | Cat#T8154 |
| Buffer TCL | QIAGEN | Cat#1031576 |
| Beta-mercaptoethanol | Sigma | Cat#M6250 |
| Humulin R | Walgreens | Cat#Hi-210 |
| Paraformaldehyde | Fisher Scientific | Cat#50980488 |
| Dichloromethane | Fisher Scientific | Cat#NC0713665 |
| Xylenes | Fisher Scientific | Cat#AC396930010 |
| Hydrogen peroxide | Fisher Scientific | Cat#501657227 |
| Glycine | Sigma | Cat#G7126 |
| DMSO | Sigma | Cat#D8418 |
| Heparin | Sigma | Cat#H3393-50KU |
| Tween-20 | Sigma | Cat#P9416 |
| Dibenzyl Ether | Sigma | Cat#33630 |
| RLT Buffer | QIAGEN | Cat#79216 |
| GoTaq qPCR master mix | Promega | Cat#A6001 |
| ProLong Gold Antifade Moutnant | Life Technologies | Cat#P36930 |
| Critical Commercial Assays | ||
| mMessage mMachine kit | Life Technologies | Cat#AM1344 |
| High-Select Fe-NTA Phosphopeptide Enrichment Kit | ThermoFisher Scientific | Cat#A32992 |
| Pierce High pH Reversed-Phase Peptide Fractionation Kit | ThermoFisher Scientific | Cat#84868 |
| SmartSeq 2 | Illumina | |
| RNA scope multiplex v2 kit | Advanced Cell Diagnostics | Cat#323100 |
| RNAscope 4-Plex Ancillary Kit for Multiplex Fluorescent V2 | Advanced Cell Diagnostics | Cat#323120 |
| RNAeasy Micro Kit | QIAGEN | Cat#74004 |
| High capacity cDNA reverse transcription kit | Fisher Scientific | Cat# 4368813 |
| Experimental Models: Cell Lines | ||
| C2C12 cells | ATCC | Cat#CRL-1772 |
| Muscle satellite cells | This study | N/A |
| Experimental Models: Organisms/Strains | ||
| C57BL/6J wild-type male mice | The Jackson Laboratories | Cat#000664 |
| SUCNR1 KO male mice | Mills et al., 2018 | N/A |
| Xenopus laevis oocytes | EcoCyte Biosciences | N/A |
| Oligonucleotides | ||
| MCT1 siRNA, SASI_Mm01_00112354 | Sigma | Cat#NM_009196 |
| MCT2 siRNA, SASI_Mm01_00096521 | Sigma | Cat#NM_011391 |
| MCT4 siRNA, SASI_Mm01_00119745 | Sigma | Cat#NM_001038653 |
| Sucnr1 FW 5′- GCAGAATGGCACAGAATTTATCT- 3′ | IDT | N/A |
| Sucnr1 Rev 5′- ACATTCCCAAGCAGTCCAA- 3′ | IDT | N/A |
| Gapdh FW 5′-AGGTCGGTGTGAACGGATTTG-3′ | IDT | N/A |
| Gapdh Rev 5′-TGTAGACCATGTAGTTGAGGT-3′ | IDT | N/A |
| Recombinant DNA | ||
| SLC16A1_OHu17492C_pcDNA3.1(+) | GenScript | Cat#OHu17492C |
| Software and Algorithms | ||
| Xcalibur | ThermoFisher Scientific | Cat#OPTON-30965 |
| TraceFinder | ThermoFisher Scientific | Cat#OPTON-30688 |
| R version 3.5.2 | R Project | https://www.r-project.org |
| RStuido Version 1.2.1335 | R Studio Team | https://rstudio.com |
| ImageJ | NIH | https://login.ezp-prod1.hul.harvard.edu/login?qurl=https://imagej.nih.gov%2fij%2f |
| GORILLA | Eden et al., 2007, 2009 | http://cbl-gorilla.cs.technion.ac.il |
| Prism 8 | GraphPad | https://www.graphpad.com/scientific-software/prism/ |
| Enrichr | Chen et al., 2013; Kuleshov et al., 2016 | https://amp.pharm.mssm.edu/Enrichr/ |
| Cufflinks version 2.2.1 | Trapnell et al., 2012 | https://github.com/cole-trapnell-lab/cufflinks |
| Imaris Version 9.3.1 | Bitplane | N/A |
| Other | ||
| Bicycle ergometer | Monark Exercise | Cat#839E |
| Animal Treadmill | Columbus Instruments | Cat# 1050-RM |
| 20 μm nylon mesh filter | EMD Millipore | Cat# NY2004700 |
| Heparin columns | Becton Dickinson | Cat# 365985 |
| Luna 5 μm NH2 column | Phenomenex | Cat#00F-4378-B0 |
| Q-Exactive HF-X mass spectrometer | ThermoFisher Scientific | Cat#0726042 |
| WPS-3000TBFC Biocompatible Well Plate Autosampler | ThermoFisher Scientific | Cat#5841.0020 |
| TCC-3000RS Thermostatted Column Compartment | ThermoFisher Scientific | Cat#5730.0000 |
| LPG-3400RS Quaternary Pump w/Degasser | ThermoFisher Scientific | Cat#5040.0036 |
| Ultrasound machine | Philips | Affiniti70 |
| Master Screen CPX System | Becton Dickinson | N/A |
| Hypoxia chamber | Coy Lab Products | N/A |
| Orbitrap Fusion Lumos Mass Spectrometer | ThermoFisher Scientific | Cat#IQLAAEGAAPFADBMBHQ |
| Orbitrap Eclipse mass spectrometer | ThermoFisher Scientific | Cat#FSN04-10000 |
| Multi-Therm heat-shake | Sigma-Aldrich | Cat#Z755753 |
| Sep-Pak C18 Cartridges | Waters | Cat#WAT054955 |
| TissueLyser II | QIAGEN | Cat#85300 |
| EASY-nLC 1200 System | ThermoFisher Scientific | Cat#LC140 |
| FAIMSPro | ThermoFisher Scientific | Cat#FMS02-10001 |
| FACS Aria II | Becton Dickinson | Cat# 643181 |
| Glucometer, ultra mini | One Touch | N/A |
| In-cage running wheels | Starr Life | N/A |
| Force Meter | Chatillon | Cat#DFE-002 |
| Nanodrop 2000 | Thermo Fisher | Cat#ND-2000 |
| Quant Studio 6 Flex Real-Time PCR machine | Applied Biosciences | Cat# 44-856-99 |