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. 2021 Jan 2;9:1. doi: 10.1186/s40168-020-00939-1

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — UPDx with double digestion increases the number of parasite-derived reads recovered following TADS. Restriction enzyme digestion yields a substantial increase in the percentage of parasite-derived 18S rDNA reads from parasite-infected whole blood samples following PCR and TADS. The increase in parasite reads is most pronounced in samples undergoing both restriction Digest 1 and Digest 2, as evidenced by the percentage of parasite-derived reads recovered from blood samples containing apixomplexans (a), kinetoplastids (b), and microfilariae (c), (statistical analysis involved a 2-way ANOVA with Tukey’s multiple comparisons posttest, **** p < 0.0001, *** p < 0.001, ** p < 0.01, n = 3, mean ± SD)