Table 4.

Detection of acute clinical T. cruzi infections using UPDx with double digestion

Sample	Tc reads^a	Total reads	% Reads	Result
Culture^b	4496	12,999	34.6%	+
Acute1	64	8549	0.7%	+
Acute2	0	4900	0.0%	−
Acute3	9189	10,731	85.6%	+
Acute4	10,864	13,428	80.9%	+
Acute5	9152	11,161	82.0%	+
Reactivation1	0	7902	0.0%	−
Reactivation2	0	6372	0.0%	−
Reactivation3	0	9592	0.0%	−
Reactivation4	0	9822	0.0%	−
Reactivation5	0	8680	0.0%	−

^aThis is the sum of reads used to construct haplotypes that were identical to T. cruzi reference sequences AF288661.1 and/or CP015675.1

^bPositive control