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. Author manuscript; available in PMC: 2022 Jan 15.
Published in final edited form as: Virus Res. 2020 Nov 6;292:198224. doi: 10.1016/j.virusres.2020.198224

Figure 4. A fluorescent rcccDNA reporter exhibits knockdown after CRISPR targeting.

Figure 4.

Green fluorescent protein (GFP) was cloned into the HBsAg ORF abolishing the overlapping polymerase reading frame. The final molecule after rcccDNA formation is depicted. (B,C) 293T cells were transfected with equal amounts of pCre/LoxP-HBV-GFP(S) and pCas9-gRNA21 or control plasmid. At 48 hours post-transfection, expression of GFP could be detected in 293T cells with control plasmid (B), whereas transfection with pCas9-gRNA21 revealed greatly reduced GFP expression (C).