Fig. 3.

GluN2B receptors mediate the effects of estradiol on generalized fear.

A. Local infusion site verification for the dCA1 and ACC. Black dots represent infusion sites for one or more animals. Drawings are adapted from Swanson, (2018). B. Schematic of the experimental procedure. All animals were trained in an inhibitory avoidance chamber. Twenty-four hours after training animals received peripheral injections of either estradiol or vehicle. Twenty-four hours after injections animals received local infusions of either Ro 25–6981, or vehicle and were then tested for generalization in either the training or the neutral context. C. Estradiol treatment alone induced contextual fear generalization when compared to vehicle-treated animals. Estradiol-induced generalization was blocked when GluN2B receptors were blocked in the dCA1. D. Estradiol treatment induced contextual fear generalization when compared to vehicle-treated animals. Estradiol-induced generalization was reduced when GluN2B receptors were blocked in the ACC. Data are expressed as mean ± SEM *p < 0.05 **p < 0.01.