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. 2020 Dec 29;11(52):4822–4835. doi: 10.18632/oncotarget.27850

Figure 2. Comparison of transcription levels of glycosyltransferases involved in the biosynthesis of Lewis glycans between high- (LN229, T98G) and low-grade (SW1088, Hs683) glioma cell lines.

Figure 2

The mRNA levels were analyzed by qRT-PCR. The relative amount of mRNA levels was normalized to the endogenous ACTB gene (human β-actin) expression and the cell line Hs683 was used as the reference sample. A significant biological result was considered at threefold difference between samples values. Data represent means ± S.D. of three independent experiments (*** p < 0.001, ANOVA followed by Tukey’s multiple comparisons test).