Figure 4. Evaluation of adhesion and migration capacities in high- and low-grade glioma cells under downmodulation of N- and core 2 O-GalNAc glycans.

(A–D) LN229 cell adhesion measured after 24 h of treatment with 150 nM TM/100 nM SW or after 48 h of MGAT5/C2GNT1 silencing. (E–H) LN229 transwell cell migration in previously incubated with 150 nM TM/100 nM SW for 24 h or after 48 h of MGAT5/C2GNT1 silencing. (I–L) SW1088 adhesion and migration measured after treatment with 150 nM TM or 100 nM SW for 24 h. Data represent means ± S.D. of three independent experiments (ns p > 0.05, ^* p < 0.05, ^** p < 0.01, ^*** p < 0.001, T Test).