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. 2020 Dec 21;11:612936. doi: 10.3389/fmicb.2020.612936

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Copyright © 2020 Hammond, Reinsel, Grinstead, Lockhart, Jordan and Mollov.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Reverse-transcription polymerase chain reaction products of assays to confirm the deletion of most of the TGB1 reading frame in ButMV-B, with RNA extracted from the original plant with the mixed infection. Lane 1—Products of degenerate primer pair ButRepFdeg/ButTGBRdeg which amplify fragments of 1,212 bp from ButMV-A, and 613 bp from ButMV-B; Lane 2—Products of ButRepFdeg paired with ButMV-A-specific reverse primer ButA-TGB, yielding an amplicon of 1,293 bp; Lane 3—Products of ButRepFdeg paired with ButMV-B-specific reverse primer ButB-TGB, yielding an amplicon of 693 bp. Lane M = 1 kb Marker ladder.