Table 1.

Initial BLASTn and final identifications of origins of random PCR products.

Random PCR product, Acc. No.	Size (nt)	Initial identification	Query coverage, isolate	Query % identity, Acc. No.	Final NGS sequence identification	% identity to NGS sequence
RdRp-a MW226001	1,026	ButMV	99%a ButMV-J	76.27 NC_013527	ButMV-B	99.81
RdRp-b MW226002	950	GLV	97% (DSM)b	73.43c KJ415259	HelVS-V	99.58
RdRp-c MW226003	585	ButMV	99% ButMV-J	79.79 NC_013527	ButMV-A	99.49
RdRp-d MW226004	439	GLVd	65% (DSM)	76.21 KJ415259	HelVS-V	99.54
CP-a MW225999	1,385	ButMV	99% ButMV-J	79.23 NC_013527	ButMV-A	99.71
CP-b MW226000	775	ButMV	99% ButMV-J	81.63 NC_013527	ButMV-B	99.87

^aPairwise alignment of RdRp-a and RdRp-c revealed 74.40% identity over the full length of RdRp-c.

^bDeutsche Sammlung von Mikroorganismen (DSM) Gaillardia latent virus isolate 5/18-05-2010.

^cSeparate pairwise alignment with the ButMV-J sequence AB517596 revealed only 59% coverage at 71.01% identity. Pairwise BLASTn of RdRp-b against the partial RdRp sequence of the Impatiens isolate of HelVS revealed an overlap of the 3′-terminal 177 nt of RdRp-b with the 5′ FJ555524 at 92.39% identity.

^dA pairwise BLASTn revealed no significant similarity to ButMV-J, but comparing RdRp-b and RdRP-d revealed a direct overlap (100% identity) of the 3′-terminal 86 nt of RdRp-d with the 5′-terminal region of RdRp-b, supporting their origin from the same source.