Table 2.
Nucleotide identities of HeLVS sequences obtained from two Veronica sources and different methods, and compared to available GenBank accessions.
| Sequence, type, length, GenBank accession no. | HelVS-VR21a (MN isolate), NGS, 8,668 nt, MW207172 | HelVS-Ver (MD isolate, ‘Sunny Border Blue’), NGS, 8,615 nt, MW246808 |
|---|---|---|
| HelVS-VR21, NGS, 8,668 nt, MW207172 | 98.58% | |
| HelVS-Va, PCR clone assembly, 4,138 nt, MW246812 | 99.76% | 98.66% |
| HelVS-Ver, NGS, 8,615 nt, MW246808 | 98.58% | |
| HelVS-Ver, PCR validation, 8,460 nt, MW246809 | 98.59% | 99.95% |
| HelVS-I, partial RdRp and TGB1, 935 nt, FJ555524b | 94.98% | 94.93% |
| HelVS, CP, and NABP, 1,389 nt, D10454c | 98.13% | 97.95% |
| HelVS, partial TGB2, TGB3, 381 nt, S71594c | 97.64% | 97.90% |
a
HelVS-VR21 and HelVS-V are, respectively, the NGS-determined, and PCR-assembled, sequences of the MN isolate termed HelVS-V.
b
HelVS-I, isolate from Impatiens, from the Netherlands.
c
HelVS, original Helenium isolate, from the United Kingdom.