Skip to main content
. Author manuscript; available in PMC: 2021 Jan 4.
Published in final edited form as: Nat Protoc. 2020 Apr 29;15(6):1922–1953. doi: 10.1038/s41596-020-0314-8
Reagent Amount (μl) Final concentration in mix
Reverse transcribed aRNA (step 127) 10.5
Nuclease-free water 10.5
2x NEBNext High-Fidelity PCR Master Mix 25 1.26 x
RNA PCR primer RP1 (10 μM) 2 0.5 μM
RNA PCR index primer RPi (10 μM) 2 0.5 μM
Total volume of reaction 50