| Reagent | Amount for 1 well (nl) | Amount for 1 plate (μl) | Final concentration in mix |
|---|---|---|---|
| Nuclease-free water | 177 | 108.5 | |
| 10x CutSmart buffer | 23 | 14.1 | 1 x |
| Dpnl (20 U/μl) | 30 | 18.4 | 0.4 U/μl |
| Total volume | 230 | 141 |
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