Reagent | Amount (μl) | Final concentration in mix |
---|---|---|
aRNA (step 125) | 5 | |
randomhexRT primer (20 μM) | 1 | 13.33 mM |
dNTP mix (10 mM) | 0.5 | 3.33 mM |
Total volume of reaction | 6.5 |
Reagent | Amount (μl) | Final concentration in mix |
---|---|---|
aRNA (step 125) | 5 | |
randomhexRT primer (20 μM) | 1 | 13.33 mM |
dNTP mix (10 mM) | 0.5 | 3.33 mM |
Total volume of reaction | 6.5 |