Figure 4.

Amplification of the canonical Cf-9, 9DC, and Cf-4 region in geographically distinct populations of S. chilense. (a) The CS5-CS1 primer pair was used to amplify a fragment of Cf-9 (378 bp), the DS1-CS1 primer pair for 9DC (507 bp), and the PSK047/PSK050 primer pair for Cf-4 (728 bp). As a PCR control, part of the coding region of elongation factor 1 alpha (EF-1α) was amplified using the primer pair RS003/RS004 (400 bp). MM-Cf-9 and MM-CF-4, and LP12 (9DC) were used as controls (electronic supplementary material, figure S2). + or – are indicative of Avr9 and Avr4 responsiveness upon infiltration of these effectors (figure 2). (b) Map shows the populations used in the analysis. (Online version in colour.)