Figure 4.

D²P² tool was used to assess the intrinsically disordered regions (IDRs) in the following S/MARBPs: CTCF, SATB1, SMAR1, PARP1, MeCP2, nucleolin, HMG1, and Ku70. It uses the following tools for prediction of disordered regions: PONDR VL-XT, PONDR VSL2b, PrDOS, Espritz, PV2, IUPred, and ANCHOR. The pastel-colored blocks (disorder predictions) are aligned and stacked against the polypeptide chain in black. The SCOP (structural classification of proteins) domains are represented by the brightly colored rounded blocks. The agreement level across the different predictors is displayed by color intensity in aligned bar and stacked below the predictions. The yellow blocks having zigzag infills represent the ANCHOR-binding region predictions along with PTM sites predicted by PhosphoSitePlus.