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. 2020 Dec 21;8:603688. doi: 10.3389/fcell.2020.603688

Figure 6.

Figure 6

ATE1 is requited for the biogenesis of succinate dehydrogenase (mitochondrial complex II). (A) The protein levels of SDHA, SDHB, SDHC, SDHD in purified mitochondria from WT and ATE1-KO MEF. TOM20 or VDAC was used as a loading control. The bar graphs represent the mean ± SEM of three independent measurements (n = 3) for SDHA, SDHB, and SDHC, or five independent measurements (n = 5) for SDHD. The p-value was calculated by t-test. (B) Similar to (A), except that whole cell lysates were used for analysis. Beta-actin was used as a loading control. The quantified results in the charts are shown on the lower panels. The bar graphs represent the mean ± SD of three independent measurements (n = 3). The p-value was calculate by t-test. (C) The mRNA level of SDHA, SDHB, SDHC, and SDHD in WT and ATE1-KO MEF measured by quantitative PCR. The β-ACTIN (ACTB) mRNA levels were used as loading control. In each testing group containing 4 replicates (n = 4), one sample from the WT cells was defined as 1.0 for the normalization of other samples. The bar graphs represent the mean ± SD. The p-value was calculated by t-test.