Figure 5.

NF-κB activation by EVs relied on a dynamin-dependant endocytosis. (A) NF-κB activation by 1 µg.ml⁻¹ EVs for 24 h in T84 cells pre-treated 2 h before with 20µM dynasore (N = 3). (B) NF-κB activation by 1 µg.ml⁻¹ EV for 24 h in T84 cells pre-treated 2 h before with endocytosis inhibitors wortmannin 20 µM, genistein 50 µM, chlorpromazine 5 µM, pitstop2 20 µM, or Cdc42 inhibitor casin 10 µM (N ≥ 3). Data are expressed as median ± quartiles of fold change toward unstimulated cells.