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. 2020 Dec 21;14:575459. doi: 10.3389/fnins.2020.575459

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Copyright © 2020 Noonong, Sobhon, Sroyraya and Chaithirayanon.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Timeline of th eexperimental procedure. Mice were randomly assigned to eight groups (n = 20 per group) consisting of two control [normal saline solution (NSS) control group and Holothuria scabra (HS) control group] and six study groups. The mice groups III to VIII were managed to subsequent behavioral tests after completed with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) treatment at day 0 and day 3. HS extract was dissolved in NSS (0.9% NaCl). Mice were euthanized by cervical dislocation.