FIGURE 6.

(A) Effects of HS on MPP⁺-induced DAergic-like neuron apoptosis shown by Hoechst 33258 stain; upper panel indicates DNA condensation in the nucleus. (B) Histograms showed percentage of apoptotic cells. (C) Expression levels of Bax, Bcl-2, and cleaved caspase 3 protein as determined by Western blot (analysis; actin was used as the control and for normalizing the intensities of the target proteins in untreated, treated with 500 μM MPP⁺, or pretreated with 1 μg/ml of HS then followed by 500 μM MPP⁺. (D) Histograms showed the relative intensity of Bax, Bcl-2, and cleaved caspase 3 expressions as normalized by actin. (E) Expression levels of tyrosine hydroxylase (TH) and α-synuclein in MPP⁺-induced DAergic-like neurons by Western blot, comparing between the untreated control, treated with 500 μM MPP⁺, treated with 1 μg/ml of HS and 500 μM MPP⁺. (F) Histograms showed the relative intensity of TH expression as normalized by action. (G) Histograms showed the relative intensity of α-synuclein expression as normalized by action. Data are presented as the means ± standard error of the mean (SEM) ^∗P < 0.05, and ^∗∗∗P < 0.001, in comparison with the untreated group. ^##P < 0.01, and ^###P < 0.001, in comparison with the MPP⁺-treated group.)