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. 2020 Dec 26;29:e00583. doi: 10.1016/j.btre.2020.e00583

Fig. 4.

Fig. 4

(A) Analysis of PCR products from the assembly and amplification of bLFcin, hLFcin, cLFcin, and ConLFcin genes on 2% agarose gel electrophoresis. Lane M points to GeneRuler 100 bp DNA marker, Lane 1 is assembly product of bLFcin, Lane 2 is amplified product of bLFcin (90 bp), Lane 3 is assembly product of hLFcin, Lane 4 is amplified product of hLFcin (90 bp), Lane 5 is assembly product of cLFcin, Lane 6 is amplified product of cLFcin (90 bp), Lane 7 is assembly product of ConLFcin, Lane 8 is amplified product of ConLFcin (90 bp). (B) Analysis of PCR products for positive transformants of pET-bLFcin, pET-hLFcin, pET-cLFcin, and pET-ConLFcin on 1.5 % agarose gel electrophoresis. Lane M points to GeneRuler 100 bp DNA marker, Lane 1 is negative transformant, Lane 2 is PCR product for positive transformant of pET-bLFcin, Lane 3 is PCR product for positive transformant of pET-hLFcin, Lane 4 is PCR product for positive transformant of pET-cLFcin, and Lane 5 is PCR product for positive transformant of pET-ConLFcin.