. 2020 Dec 26;29:e00583. doi: 10.1016/j.btre.2020.e00583

Table 2.

Physico-chemical properties of the bLFcin, hLFcin, cLFcin, and ConLFcin peptides.

Property	bLFcin	hLFcin	cLFcin	ConLFcin
Number of amino acids	30	30	30	30
Number of amino acids	36^*	36^*	36^*	36^*
Molecular weight (Daltons)	3655.47	3576.28	3542.26	3737.58
Molecular weight (Daltons)	4478.31^*	4399.12^*	4365.11^*	4560.42^*
Theoretical pI	10.90	10.33	10.57	11.63
Total number of positively charged residues (Aspartic acid (D) + Glutamic acid (E))	8	7	9	11
Total number of negatively charged residues (Arginine (R) + Lysine (K))	1	1	1	1
Total number of atoms	520	504	503	535
Total number of atoms	622^*	606^*	605^*	637^*
Aliphatic index	71.67	58.33	45.33	48.67
Aliphatic index	59.72^*	48.61^*	37.78^*	40.56^*
Grand average of hydropathicity (GRAVY)	−0.177	−0.717	−0.787	−0.863
Grand average of hydropathicity (GRAVY)	−0.681^*	−1.131^*	−1.189^*	−1.253^*
+Estimated half-life	−1.1 h (mammalian reticulocytes, in vitro).	−7.2 h (mammalian reticulocytes, in vitro).	−1.9 h (mammalian reticulocytes, in vitro).	−1.9 h (mammalian reticulocytes, in vitro).
	−3 min (yeast, in vivo).	- >20 h (yeast, in vivo).	- >20 h (yeast, in vivo).	- >20 h (yeast, in vivo).
	−2 min (Escherichia coli, in vivo).	- >10 h (Escherichia coli, in vivo)	- >10 h (Escherichia coli, in vivo).	- >10 h (Escherichia coli, in vivo).
∼Instability index	81.25	55.90	44.28	80.60
∼Instability index	72.80^*	51.68^*	42.00^*	72.26^*
Atomic Formula	C₁₆₄H₂₆₅N₅₁O₃₆S₄	C₁₅₄H₂₅₆N₅₀O₄₀S₄	C₁₅₁H₂₅₈N₅₀O₄₀S₄	C₁₆₃H₂₇₅N₅₇O₃₆S₄
Atomic Formula	C₂₀₀H₃₀₇N₆₉O₄₂S₄^*	C₁₉₀H₂₉₈N₆₈O₄₆S₄^*	C₁₈₇H₃₀₀N₆₈O₄₆S₄^*	C₁₉₉H₃₁₇N₇₅O₄₂S₄^*

+Estimated half-life: N-terminal of the hLFcin, bLFcin, cLFcin, and ConLFcin sequences are C (Cysteine).

∼Instability index of the hLFcin, bLFcin, cLFcin, and ConLFcin peptides is computed to be 55.90, 81.25, 44.28, and 80.60, respectively. This classifies the peptides as unstable.

Calculated values for peptides tagged with 6xHis-tag. The remaining data did not change after tagging of peptides.