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. 2020 Dec 29;6(12):e05810. doi: 10.1016/j.heliyon.2020.e05810

Figure 1.

Figure 1

Flow cytometry analysis of mouse cells isolated from frozen heart tissue samples pretreated with an RNA-stabilizing agent. Murine heart tissue was digested with (A) collagenase type II, (B) collagenase type I and hyaluronidase, (C) elastase, (D) Liberase, or (E) collagenase type II and elastase, and analysed by flow cytometry and high content screening (HCS). Isolated cells were identified and gated first by forward scatter area (FSC-A) vs DRAQ5, second on FSC-A vs FSC-height (FSC-H) to distinguish single cells. The morphology and integrity of DRAQ5-stained cell suspensions were analysed using a HCS fluorescent microscope (scale bar = 200 μm). Prior to digestion, hearts had been treated with an RNA-stabilizing agent and frozen to -80 °C.