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Flow cytometry analysis of a second sample from the same human heart. (A) Nuclei were first identified by forward scatter area (FSC-A) vs side scatter area (SSC-A). (B) In the second gate DRAQ5 positive nuclei were separated from debris. (C) Single nuclei were gated by SSC-height (SSC-H) versus SSC-width (SSC-W). (D) Cardiomyocyte nuclei were separated from non-cardiomyocyte nuclei based on PCM1-staining.
