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. 2020 Dec 29;6(12):e05810. doi: 10.1016/j.heliyon.2020.e05810

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© 2020 The Author(s)

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Flow cytometry analysis of mouse cells isolated from fresh heart tissue. Murine heart tissue was digested with (A) collagenase type II, (B) collagenase type II and hyaluronidase, (C) elastase, (D) Liberase, or (E) collagenase type II and elastase, and analysed by flow cytometry and high content screening (HCS). Isolated cells were identified and gated first by forward scatter area (FSC-A) vs DRAQ5, second on FSC-A vs FSC-height (FSC-H) to distinguish single cells. The morphology and integrity of DRAQ5-stained cell suspensions were analysed using a HCS fluorescent microscope (scale bar = 200 μm).