Figure 5.

Evaluation of nuclei isolated from human cardiac left ventricle tissue. (A) Nuclei were first identified by forward scatter area (FSC-A) vs side scatter area (SSC-A). (B) In the second gate DRAQ5 positive nuclei were separated from debris. (C) Single nuclei were gated by SSC-height (SSC-H) versus SSC-width (SSC-W). (D) Cardiomyocyte nuclei were separated from non-cardiomyocyte nuclei based on PCM1-staining. (E,F,G) Gene expression of sorted nuclei revealed that the PCM1⁺ fraction was enriched for the cardiomyocyte marker genes PCM1 and TNNT3, whereas the cardiac fibroblast marker gene VIM was enriched in the PCM1^- fraction. Data is presented as mean ± SD from three independent experiments. (H) FACS-sorted nuclei, stained with DRAQ5 and PCM1 were scanned with high content screening microscopy. Scale bar = 10 μm.