Figure 7.

RNA-sequencing of human heart cells. Panel A shows a Uniform Manifold Approximation and Projection (UMAP) plot based on RNA-sequencing of 8,460 individual nuclei from a human heart. Of the nuclei, the proportion of cardiomyocyte nuclei has been enriched such that 4,390 had been selected from PCM1⁺ and 4,070 from PCM1^- nuclear fractions by fluorescence-activated cell sorting. Panel B shows a dot plot of expression across clusters of preselected, established marker genes representing the major expected cell types in human heart: cardiomyocytes (red), fibroblasts (yellow), endothelial cells (green), vascular smooth muscle cells and pericytes (cyan), macrophages (blue), lymphocytes (purple), and neurons (pink).