Figure 9.

Clustering of nuclei by PCM1-staining. Uniform Manifold Approximation and Projection (UMAP) projections based on RNA-sequencing of 4,070 PCM1^- (panels A and B) and 4,390 PCM1⁺ (C and D) nuclear fractions from fluorescence-activated cell sorting. Below UMAP plots are corresponding dot plots (panels B and D) illustrating expression across clusters of preselected, established marker genes representing the major expected cell types in human heart, with color codes representing the PCM1^- clusters: cardiomyocytes (cyan), fibroblasts (red), endothelial cells (yellow), vascular smooth muscle cells and pericytes (light green), leukocyte populations (dark green), neurons (blue) and adipocytes (pink).