A. NeuN, HuC/D, MAP2, and TUBB3 staining of P7 and P14
Utx+/+ and UtxF/F
Atoh1-Cre SmoM2 cerebella showed significantly reduced neuron
numbers in UTX deleted samples.
B. BrdU tracing (24 hours) experiments revealed decreased
differentiation in UtxF/F Atoh1-Cre SmoM2
precancerous CGNPs at P14 than the wildtype CGNPs. The neuron differentiation
rate was quantified as the percentage of HuCD+ BrdU+ cells
in total BrdU+ cells.
Student’s t-test, **, p<0.01.