Figure 1.

Effects of Ccr2 or Ccr5 deficiency on fibrosis, steatosis, and tumorigenesis in NEMO LPC-deficient mice. (A) Eight-week-old NEMO^WT, NEMO^LPC-KO, NEMO^LPC-KOCcr2^–/–, and NEMO^LPC-KOCcr5^–/– mice were analyzed for histology using hematoxylin and eosin staining and for (B) liver injury reflected by ALT after 8 and 12 weeks of age. (C) Hepatic inflammation assessed based on the expression of inflammatory mediators in whole liver. (D) Fibrosis quantification based on Sirius Red microscopy of liver sections from 8-week-old mice. (E) Sirius Red area quantification, (F) hepatic hydroxyproline content after 8 and 12 weeks, and (G) expression of fibrosis-related mRNA in liver tissue. (H) Hepatic tumors at the 26 weeks of age (representative macroscopic pictures of 26-week-old mice), (I) as quantified by the cumulated diameter of tumors and tumors larger than 1 mm. Data represent median of n = 3–6; ∗P < .05, ∗∗∗P < .001 (1-way analysis of variance).