Figure 10.

Amelioration of liver injury in NEMO^LPC-KOCcr5^–/– mice with adoptive macrophage cell transfer. NEMO^LPC-KOCcr5^–/– mice at the age of 8 weeks were treated with CLLs and received 5 hours later CD115⁺ cells from bone marrow (immature monocytes [IMMs]), B cells (from spleen), and BMDMs. Mice were sacrificed after another 11 hours. (A) Experimental setup for adoptive cell transfer. (B) In blood, 7% of CD45⁺ leukocytes were FITC⁺, and the majority (45%) were myeloid cells. (C) Bead-bearing cells accumulated in liver with 5% of liver leukocytes containing beads. The FITC(beads)⁺ cells at 98% F4/80⁺ macrophages (middle graphics) showed either markers of CD11b⁺ F4/80^int monocytic macrophages (MoMFs) or F4/80⁺CD11b^– (KCs). (D) Liver histology after the cell transfers and (E) corresponding ALT levels as a measure of liver injury in the different treatment conditions. Data represent median of n = 4–6 ± SD; ∗P < .05, ∗∗P < .01, ∗∗∗P < .001 (1-way analysis of variance).