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. 2020 Sep 4;11(2):327–347. doi: 10.1016/j.jcmgh.2020.08.012

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© 2020 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Effects of Ccr2 or Ccr5 deficiency on liver metabolism and cell death in NEMO LPC-deficient mice. Eight-week-old WT, NEMO^LPC-KO, NEMO^LPC-KOCcr2^–/–, and NEMO^LPC-KOCcr5^–/– mice were analyzed for (A) steatosis using Oil red O staining. (B) Oil red O quantifications. (C) Analysis of free fatty acids from liver tissue. (D) Assessment of triglycerides levels from liver tissue. (E) Expression of metabolism-related mRNA in liver tissue. (F) TUNEL staining for apoptotic cell death and (G) quantification. (H) Expression of selected mRNA related to cell death. Data represent median of n = 3–7; ∗P < .05 (1-way analysis of variance).