Figure 4.
Impact of CCR2 and CCR5 on hepatocyte viability and HSC activation. (A) Cell culture micrographs of primary hepatocytes from NEMOWT, NEMOLPC-KO, NEMOLPC-KOCcr2–/–, and NEMOLPC-KOCcr5–/– mice 24 hours after isolation. (B) quantification of hepatocyte viability by MTT assay. (C) HSCs were isolated from healthy WT or Ccr5–/– mice at the age of 8 weeks. Representative micrographs of HSC after 3 days of culture, with or without stimulation for 24 hours using LPS at 100 ng/mL, PDGF, and TGF-β. (C) Changes in CCL2 and IL6 cytokine release by HSCs as monitored in the cell culture medium after 24 hours. Data represent median of n = 3–6; ∗P < .05, ∗∗P < .01, ∗∗∗P < .001 (1-way analysis of variance).