Figure 5.

Effects of Ccr2 or Ccr5 deficiency on hepatic immune cell infiltration in NEMO LPC-deficient mice. NEMO^LPC-KO, NEMO^LPC-KOCcr2^–/–, and NEMO^LPC-KOCcr5^–/– mice were analyzed at the age of 8 weeks. (A) Immunohistochemical staining of the pan-leukocyte marker CD45 and (B) quantification of liver leukocytes by flow cytometry. (C) Macrophage staining based on F4/80 and (D) quantification of liver macrophages by flow cytometry. (E) Analysis of hepatic macrophage subsets, mainly CD11b⁺F4/80^int monocytic macrophages (MoMF, red gate) and CD11b^intF4/80⁺ KCs (green gate), and (F) quantification of MoMFs. (G) Flow cytometric analysis of markers of alternative macrophage activation expressed by MoMFs, and (H) quantifications thereof. (I) Representative flow cytometric plots of hepatic lymphoid cells (blue gate: NK cells), and (J) statistical summary of cell frequencies. Data represent median of n = 2–14; ∗P < .05, ∗∗P < .01, ∗∗∗P < .001 (1-way analysis of variance).