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. 2020 Sep 4;11(2):327–347. doi: 10.1016/j.jcmgh.2020.08.012

Figure 9.

Figure 9

Functional role of myeloid immune cells for modulating liver injury in NEMO-LPC-deficient mice. Specific immune cell subsets were depleted in vivo using monoclonal antibodies or macrophage-depleting CLL in WT, NEMOLPC-KO, NEMOLPC-KOCcr2–/–, and NEMOLPC-KOCcr5–/– mice at 8 weeks of age. (A) Reduction of CD11b+ myeloid cells by 100 μg of the antibody RB6-8C5 significantly increased liver injury in NEMOLPC-KO mice as shown by flow cytometry. (B) CLLs led to a potent depletion of F4/80+ cells in the liver as shown by flow cytometry and (C) F4/80 immunohistochemistry. (D) CLLs significantly increased hepatic injury in NEMOLPC-KO, NEMOLPC-KOCcr2–/–, and NEMOLPC-KOCcr5–/– mice. (E) Effects of CLLs on selected hepatic cytokines. (F) Liver histology of NEMOLPC-KOCcr5–/– mice during the time course of CLL-mediated macrophage deletion. (G) Corresponding ALT levels. Data represent median of n = 3–7 ± SD; ∗P < .05, ∗∗∗P < .001 (1-way analysis of variance).