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. 2020 Dec 21;11:621654. doi: 10.3389/fmicb.2020.621654

Figure 2.

Figure 2

Tp0954-expressing bacteria bind to various mammalian cell lines. (A,D) Radiolabeled Tp0954-expressing B314 and plasmid transformed control strains were used to evaluate binding to mammalian epithelial cell lines. B314(pTp-Bb) bound at a 5-fold higher level to Vero cells (A), and 6-fold higher level to HEK293 (293) cells to (D) as compared B314 transformed by the empty pJSB175 vector, B314(V). (B,C) Binding assay conducted followed by washing and bioluminescence measurement confirmed that B314(pTP-Bb) binds at significantly higher level to 293 and C6 glioma cells. (E) B314(pTp-Bb) bound 3.6-fold higher level to the C6 glioma neuronal cells compared to control strain, while (F) B314(pTp-Bb) binds relatively weakly to endothelial EA.hy926 cell line. (G) Increase in binding of B314(pTp-Bb) was 3.5-fold higher than B314(V) to placental BeWo cell line. Statistically significant difference was determined by unpaired two-tailed t-test with Welch's correction (****P < 0.0001).