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. 2020 Dec 21;11:621654. doi: 10.3389/fmicb.2020.621654

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Copyright © 2020 Primus, Rocha, Giacani and Parveen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Heparan sulfate and dermatan sulfate as ECM components on specific mammalian cells are required for Tp0954-expressing spirochetes binding. (A,C) Low level binding of B314(V) strain were observed to both 293 and C6 glioma cells and removal of GAGs by specific lyases reduced binding further. (B) Pretreatment of 293 with chondroitinase ABC and not by a combination of heparinase I and III (HI and HIII) inhibited binding of radiolabeled Tp0954-expressing B314 strain, indicating contribution of dermatan sulfate to adherence on these cells, while (D) removal of both heparan sulfate and dermatan sulfate significantly reduced binding of B314(pTp-Bb) to C6 cell line. GAG-lyases are labeled as: NT-No Treatment, Chon-ABC-chondroitinase ABC, and HI+ HII-heparinase I + III. Statistically significant difference was determined by unpaired two-tailed t-test with Welch's correction (*P < 0.05, ****P < 0.0001, ns, not significant).