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Spontaneous contractions and size of terminal buds in E11.5 explant cultures over time. (A). Contractions were measured in WT and KO explants at starting point E11.5 and after 53 and 73 h of organ culture (B) The size of the endbuds was determined by measuring projected surface area at the start of the culture of WT, KO–, KO+ E11.5 (time 0) explants, as well as after 24, 43, and 52 h of cultivation. Data were analyzed by 2-way ANOVA and Bonferroni post-test, *P = 0.05 and **P = 0.01
