Table 4.

Thresholds for distinguishing MSIs from microevolution events as per reviewed studies.

Yeara	Methodb	Microevolutionc	MSIc	References
2004	IS6110 RFLP	1-3 different bands	>3 different bands	Shamputa et al., 2004
	MIRU-VNTR	Double allele at 1 locus	Double alleles at ≥2 Loci
2011	MIRU-VNTR	Double allele at 1 locus	Double alleles at ≥2 Loci	Cohen et al., 2011
2013	WGS	<80 heterogeneous SNPs	>80 heterogeneous SNPs	Bryant et al., 2013b
2014	IS6110 RFLP	1-2 different bands	>2 different bands	Marx et al., 2014
2015	WGS	≤140 heterogeneous SNPs	>140 heterogeneous SNPs	Guerra-Assuncaõ et al., 2015
2019	MIRU-VNTR	Double allele at 1 locus	Double alleles at ≥2 Loci	Maghradze et al., 2019
2019	WGS	0-5 heterogeneous SNPs	757-833 heterogeneous SNPs	Dippenaar et al., 2019

^aYear of publication in chronological order.

^bDetails regarding specific methods are included in the main text and Supplementary Table 2.

^cThresholds or criteria used by the respective methods to differentiate between the two forms of infection.