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. 2020 Dec 30;15:10539–10546. doi: 10.2147/IJN.S285899

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© 2020 Gao and Li.

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Optimization of the AlphaLISA sST2 working conditions. The effect of a single variable on the intensity of CL was examined by making the other factors constant. (A) Effect of the concentration of biotinylated anti-sST2 antibodies; (B) Effect of the dilution ratios of chemibeads coated with anti-sST2 antibodies; (C) Effect of incubation time. The error bars of some points were invisible because they were shorter than the height of the symbol. Low: low levels of sST2 serum pool (35.27 ng/mL); High: high levels of sST2 serum pool (141.63 ng/mL).